ABSTRACT
Objective • To reveal the effect of denatured collagen type on the endothelial cell proliferation, migration, and angiogenesis-related proteins expression. Methods • Human umbilical vein endothelial cells were cultured on the plates coated with normal collagen (normal collagen group), half concentration normal collagen (half collagen group) or denatured collagen (denatured collagen group). CCK-8 assay was performed to test cell proliferation ability two days later. The effect of collagen on cell migration was measured by scratch test. The expressions of membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor (VEGF) were detected by Western blotting. The expressions of angiopoietin 1 (Ang-1) and Ang-2 were measured by ELISA. Results • CCK-8 results showed that the proliferation activity of denatured collagen group was significantly higher than that of normal collagen group and half collagen group (P<0.05). Cell migratory capacity was increased in denatured collagen group, of which the scratch was almost covered after 16 h. The protein expressions of MT1-MMP and VEGF were significantly higher in denatured collagen group than those in the other two groups (P<0.05), in which the concentration of Ang-2 was also higher than that in normal collagen group (P<0.05). However, no significant difference was found in the concentration of Ang-1 among the three groups. Conclusion • Denatured collagen type can promote proliferation, migration and MT1-MMP, VEGF, and Ang-2 expressions in endothelial cells, suggesting that collagen denaturation may play an active role in the process of angiogenesis during wound healing.
ABSTRACT
<p><b>OBJECTIVE</b>To form a wound information management scheme with objectivity, standardization, and convenience by means of wound information management system.</p><p><b>METHODS</b>A wound information management system was set up with the acquisition terminal, the defined wound description, the data bank, and related softwares. The efficacy of this system was evaluated in clinical practice.</p><p><b>RESULTS</b>The acquisition terminal was composed of the third generation mobile phone and the software. It was feasible to get access to the wound information, including description, image, and therapeutic plan from the data bank by mobile phone. During 4 months, a collection of a total of 232 wound treatment information was entered, and accordingly standardized data of 38 patients were formed automatically.</p><p><b>CONCLUSIONS</b>This system can provide standardized wound information management by standardized techniques of acquisition, transmission, and storage of wound information. It can be used widely in hospitals, especially primary medical institutions. Data resource of the system makes it possible for epidemiological study with large sample size in future.</p>